sfk py527 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc manufactures this product 
96
Structured Review
Cell Signaling Technology Inc
sfk py527
Sfk Py527, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+py527+src/Phospho-Src+(Tyr527)+Antibody/pm20583816__cb100099h_si_001-12-21-24
Average 96 stars, based on 370 article reviews
Sfk Py527, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 370 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+py527+src/Phospho-Src+(Tyr527)+Antibody/pm20583816__cb100099h_si_001-12-21-24
Average 96 stars, based on 370 article reviews
sfk py527 - by Bioz Stars,
2026-10
96/100 stars
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Western Blot:Article Title: NAD + supplement potentiates tumor-killing function by rescuing defective TUB-mediated NAMPT transcription in tumor-infiltrated T cells. Article Snippet: Following incubation with the secondary antibody, membranes were washed with TBST for 5 mins at room temperature three times and then incubated with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher) for visualization on film. .. The following antibodies were purchased from the indicated suppliers and used for immunoblotting (unless otherwise stated) at the indicated concentrations: anti-b-actin (Sigma, cat. no. A5441, clone AC-15); anti-NAMPT (Proteintech, cat. no. 66385-1-lg); Anti-Phosphotyrosine clone 4G10 (Millipore, cat. no. 05-321); antipY493 Zap70 (Cell signaling technology, cat. no. 2704); anti-Zap70 (Cell signaling technology, cat. no. 2705); anti-pZeta (Abcam, cat. no. ab68235); anti-Zeta (Abcam, cat. no. ab190728); anti-pErk (Cell signaling technology, cat. no. 9101); anti-Erk (Cell signaling technology, cat. no. 9102); Membrane:Article Title: Effects of sex and DTNBP1 (dysbindin) null gene mutation on the developmental GluN2B-GluN2A switch in the mouse cortex and hippocampus Article Snippet: .. PSD enrichments (frontal cortex, 2 μg, or hippocampus, 4 μg) or membrane preparations (frontal cortex, 10 μg) were loaded on 7.5 % Tris-glycine polyacrylamide gels (Bio-Rad) and separated by electrophoresis at 200 V. Proteins were then transferred to Immobilon FL PVDF membranes (Millipore, Billerica, MA, USA) at 100 V for 1 h. They were cut at approximately 130 and 75 kDa (as indicated in Fig. and Additional file : Figure S2) and after blocking for 1 h in TBS blocking buffer (LI-COR Biosciences, Lincoln, NE, USA), were probed with the following primary antibodies overnight: anti-GluN1 (1:500; sc-1467, Santa Cruz Biotechnology, Dallas, TX, USA); anti-GluN2A (1:1000; sc-1468, Santa Cruz); anti-GluN2B (1:1000; 06-600, Millipore); anti-pY1472-GluN2B (1:500; #4208, Cell Signaling Technology, Danvers, MA, USA); anti-PSD-95 (1:5000; clone K28/43, UC Davis/NIH NeuroMab Facility); anti-SAP102 (1:2000; clone N19/2, UC Davis/NIH NeuroMab Facility); anti-PLCγ1 (1:250; sc-81, Santa Cruz); anti-Fyn (1:500; sc-16, Santa Cruz); anti-cSrc (1:1000; L4A1-2110S, Cell Signaling); Electrophoresis:Article Title: Effects of sex and DTNBP1 (dysbindin) null gene mutation on the developmental GluN2B-GluN2A switch in the mouse cortex and hippocampus Article Snippet: .. PSD enrichments (frontal cortex, 2 μg, or hippocampus, 4 μg) or membrane preparations (frontal cortex, 10 μg) were loaded on 7.5 % Tris-glycine polyacrylamide gels (Bio-Rad) and separated by electrophoresis at 200 V. Proteins were then transferred to Immobilon FL PVDF membranes (Millipore, Billerica, MA, USA) at 100 V for 1 h. They were cut at approximately 130 and 75 kDa (as indicated in Fig. and Additional file : Figure S2) and after blocking for 1 h in TBS blocking buffer (LI-COR Biosciences, Lincoln, NE, USA), were probed with the following primary antibodies overnight: anti-GluN1 (1:500; sc-1467, Santa Cruz Biotechnology, Dallas, TX, USA); anti-GluN2A (1:1000; sc-1468, Santa Cruz); anti-GluN2B (1:1000; 06-600, Millipore); anti-pY1472-GluN2B (1:500; #4208, Cell Signaling Technology, Danvers, MA, USA); anti-PSD-95 (1:5000; clone K28/43, UC Davis/NIH NeuroMab Facility); anti-SAP102 (1:2000; clone N19/2, UC Davis/NIH NeuroMab Facility); anti-PLCγ1 (1:250; sc-81, Santa Cruz); anti-Fyn (1:500; sc-16, Santa Cruz); anti-cSrc (1:1000; L4A1-2110S, Cell Signaling); Blocking Assay:Article Title: Effects of sex and DTNBP1 (dysbindin) null gene mutation on the developmental GluN2B-GluN2A switch in the mouse cortex and hippocampus Article Snippet: .. PSD enrichments (frontal cortex, 2 μg, or hippocampus, 4 μg) or membrane preparations (frontal cortex, 10 μg) were loaded on 7.5 % Tris-glycine polyacrylamide gels (Bio-Rad) and separated by electrophoresis at 200 V. Proteins were then transferred to Immobilon FL PVDF membranes (Millipore, Billerica, MA, USA) at 100 V for 1 h. They were cut at approximately 130 and 75 kDa (as indicated in Fig. and Additional file : Figure S2) and after blocking for 1 h in TBS blocking buffer (LI-COR Biosciences, Lincoln, NE, USA), were probed with the following primary antibodies overnight: anti-GluN1 (1:500; sc-1467, Santa Cruz Biotechnology, Dallas, TX, USA); anti-GluN2A (1:1000; sc-1468, Santa Cruz); anti-GluN2B (1:1000; 06-600, Millipore); anti-pY1472-GluN2B (1:500; #4208, Cell Signaling Technology, Danvers, MA, USA); anti-PSD-95 (1:5000; clone K28/43, UC Davis/NIH NeuroMab Facility); anti-SAP102 (1:2000; clone N19/2, UC Davis/NIH NeuroMab Facility); anti-PLCγ1 (1:250; sc-81, Santa Cruz); anti-Fyn (1:500; sc-16, Santa Cruz); anti-cSrc (1:1000; L4A1-2110S, Cell Signaling); |